Brazilian essential oil of Cymbopogon martini (Poaceae): positive effects on inflammation-induced human fibroblasts and skin aging

Carlos Rocha Oliveira
Leonardo Mendes Bella
Marília Cristina Duarte
Rodolfo de Paula Vieira

    Carlos Rocha Oliveira

    Anhembi Morumbi University, Health Sciences. Rua Doutor Almeida Lima, s/n, Mooca, CEP 03164-000, São Paulo, SP, Brazil

    Pharmacist, Master in Pharmacology and Doctor in Biotechnology (USP). Post-doctorate at the Institute of Pharmacology and Molecular Biology (UNIFESP). Professor at Universidade Anhembi Morumbi, teaching at the Medicine course and Coordinator of the Human Research Ethics Committee (CEP). He develops projects from compounds of natural origin, with an emphasis on cell signaling and the mode of action of drugs, working with in vivo models and in vitro molecular assays. Advisor at the Graduate Program in Biomedical Engineering (UNIFESP), Campus São José dos Campos-SP.

    Leonardo Mendes Bella

    Anhembi Morumbi University, Health Sciences. Rua Doutor Almeida Lima, s/n, Mooca, CEP 03164-000, São Paulo, SP, Brazil.

    Graduated in Pharmacy and Biochemistry from the Federal University of Juiz de Fora (2012), Master's in Pharmacy (Physiopathology and Toxicology) from the University of São Paulo (2014) and Ph.D. in Pharmacy (Physiopathology and Toxicology) from the University of São Paulo (2018) and a postgraduate degree in microbiology (2020) at Faculdade Oswaldo Cruz (FOC). He is currently a professor at the School of Health at Universidade Anhembi Morumbi.

    Marília Cristina Duarte

    University of Mogi das Cruzes, Center for Biomedical Sciences, Laboratory of Plant Systematics. Avenida Dr. Cândido Xavier de Almeida e Souza, 200, Centro Cívico, CEP 08780-911, Mogi das Cruzes, SP, Brazil.

    Doctor and Master in Plant Biodiversity and Environment, in the field of plant systematics, by the Institute of Botany of São Paulo. She is currently a researcher and professor at the undergraduate and graduate level at the University of Mogi das Cruzes (UMC), as well as curator of the Herbarium Mogiense (HUMC). She has experience in Botany, with an emphasis on Spermatophyte Systematics, especially from the Malvaceae family, working mainly in floristic inventories and institutional projects. she having published scientific articles, book chapters and participated in National and International Congresses of Botany.

    Rodolfo de Paula Vieira

    University Brazil, Campus Itaquera. Rua Carolina Fonseca, 235, Vila Santana, CEP 08230-030, São Paulo, SP, Brazil.

    Graduated in Physical Education (Univap 1998-2001), Master in Biological Sciences (Univap 2001-2003), Doctorate in Pathology (Scholarship CAPES, Faculty of Medicine USP 2004-2007) and Post-doctorate in Physical Activity and Pulmonary Immunopathology (Scholarship FAPESP , Faculty of Medicine USP 2008-2009), Post-doctorate in Asthma Immunology (Deutsche Forchungsgemeinschaft Contract 2009-2010) by Albert-Ludwigs University of Freiburg - Germany, Post-doctorate in Physical Activity and Pulmonary Immunopathology (European Respiratory Award Scholarship Society/Marie Curie Foundation/European Union 2010-2011). He was a guest researcher in the Department of Pulmonology at Albert-Ludwigs University of Freiburg in Germany (June 2010 to May 2011) and an associate researcher in the same department (June 2011 to December 2011). He is a researcher responsible for the project "Effects of aerobic physical training on lung changes induced by different products of atmospheric pollution" financed by the MCT / CNPq which is part of the (INCTs-National Institutes of Science and Technology). Since April/2014 he is the author and responsible for the FAPESP Young Researcher Project 2012/15165-2 entitled "Role of purinergic signaling and SOCS-JAK-STAT signaling in the anti-inflammatory effects of aerobic training in experimental models of asthma and in asthmatic individuals" , from where he obtained funds to set up the Laboratory of Pulmonary and Exercise Immunology (LABPEI) in the city of São José dos Campos. He is currently director of the Brazilian Institute of Teaching and Research in Pulmonary and Exercise Immunology (IBEPIPE). He is the coordinator of LABPEI (www.labpei.com.br), which is the functional arm of IBEPIPE, developing clinical and experimental research work, focusing on cellular and molecular immunological aspects involved both in the pathophysiology of lung diseases and in the effects of physical exercise in lung diseases. In addition, the research focus of the laboratory includes research in the field of exercise immunology, which is carried out with professional and amateur athletes and different sports teams. Permanent advisor of the master's and doctoral programs in Human Movement and Rehabilitation Sciences at UNIFESP (and collaborating professor) and the professional master's degree in Bioengineering at Universidade Brasil. He has been a CNPq Research Productivity Scholarship 2 since 2016.

Keywords

Cymbopogon martini
Anti-inflammatory
Anti-aging
Hyaluronic acid
Collagen

Abstract

The study evaluated the effects of essential oil from Cymbopogon martini (CMEO) on lipopolysaccharide (LPS)-stimulated human fibroblasts. Samples were collected in Monte Verde, Minas Gerais, Brazil and analyzed by gas chromatography with mass spectrometry. The fibroblasts were cultured in a monolayer using Iscove's medium and stimulated by LPS (1 μg/mL) and incubated for 24 h at 37°C. The cytotoxicity was evaluated by MTT assay and collagen concentration by Sirius red. Collagenase activity, hyaluronic acid, and the concentrations of IL-1β; IL-6; MCP-1 (CCL2), and MIP-1-α (CCL3) were evaluated. The effect of CMEO on the expression of mRNA and the secretion of enzymes in fibroblasts were evaluated by RT-qPCR and ELISA, respectively. CMEO was cytotoxic against fibroblasts, in which 10 μg/mL inhibited 50% of cell viability. When treated with CMEO, the fibroblasts produced more collagen and hyaluronic acid than control cells. When stimulated by LPS, fibroblasts exhibited higher production of IL-6, IL-1β, MCP-1, and MIP-1α than control cells. The study demonstrated the effects of CMEO on the modulation of mediators related to inflammation and decreasing the mRNA and secretion levels of metalloproteinases, revealing to be a promising candidate for anti-aging effects and wound healing treatments.

References

  1. Promila. A review on the medicinal and aromatic Plant Cymbopogon martini (Roxb.) Watson (Palmarosa). Inter J Chem Studies. 2018; 6(2): 1311-15. ISSN 2349-8528. [Link].
  2. Scherer R, Wagner R. Duarte MCT, Godoy HT. Composição e atividades antioxidante e antimicrobiana dos óleos essenciais de cravo-da-índia, citronela e palmarosa. Rev Bras Pl Med. 2009; 11(4): 442-49. ISSN 1516-0572. [CrossRef].
  3. Elshafie HS, Camele I. An Overview of the biological effects of some mediterranean essential oils on human health. Biomed Res Int. 2017; 2017: 9268468. [CrossRef] [PubMed].
  4. Andrade BFMT, Conti BJ, Santiago KB, Fernandes Júnior A, Sforcin JM. Cymbopogon martini essential oil and geraniol at noncytotoxic concentrations exerted immunomodulatory/anti-inflammatory effects in human monocytes. J Pharm Pharmacol. 2014; 66(10):1491-6. [CrossRef] [PubMed].
  5. Wang J, Su B, Zhu H, Chen C, Zhao G. Protective effect of geraniol inhibits inflammatory response, oxidative stress and apoptosis in traumatic injury of the spinal cord through modulation of NF-κB and p38 MAPK. Exp Ther Med. 2016; 12(6): 3607-13. [CrossRef] [PubMed].
  6. Abdulkhaleq LA, Assi MA, Abdullah R, Zamri-Saad M, Taufiq-Yap YH, Hezmee MNM. The crucial roles of inflammatory mediators in inflammation: A review. Vet World. 2018; 11(5): 627-35. E-ISSN 2231-0916. [CrossRef] [PubMed].
  7. des Jardins-Park HE, Foster DS, Longaker MT. Fibroblasts and wound healing: an update. Regen Med. 2018; 139(5): 491-95. ISSN 1746-0751. [CrossRef].
  8. Gao HM, Hong JS. Why neurodegenerative diseases are progressive: uncontrolled inflammation drives disease progression. Trends Immunol. 2008; 29(8): 357-65. [CrossRef].
  9. Raina VK, Srivastava SK, Aggarwal KK, Syamasundar KV, Khanuja SPS. Essential oil composition of Cymbopogon martini from different places in India. Flavour Fragr. J. 2003; 18(4): 312-15. [CrossRef].
  10. Prashar A, Hili P, Veness RG, Evans CS. Antimicrobial action of palmarosa oil (Cymbopogon martini) on Saccharomyces cerevisiae. Phytochemistry. 2003 Jul;63(5):569-75. doi: 10.1016/s0031-9422(03)00226-7. [CrossRef] [PubMed] PMID: 12809717.
  11. Randriamiharisoa RP, Gaydou EM. Composition of palmarosa (Cymbopogon martini) essential oil from Madagascar. J Agric Food Chem. 1987; 35(1): 62-6. ISSN 0021-8561.
  12. Oliveira PF, Alves JM, Damasceno JL, Oliveira RAM, Dias Júnior H, Crotti AEM et al. Cytotoxicity screening of essential oils in cancer cell lines. Rev Bras Farmacogn. 2015; 25(2): 183-8. [CrossRef].
  13. Elsayed EA, Sharaf-Eldin MA, Wadaan M. In vitro Evaluation of Cytotoxic Activities of Essential Oil from Moringa oleifera Seeds on HeLa, HepG2, MCF-7, CACO-2 and L929 Cell Lines. Asian Pacific J Cancer Prev. 2015; 16(11): 4671-75. [CrossRef] [PubMed].
  14. Binic I, Lazarevic V, Ljubenovic M, Mojsa J, Sokolovic D. Skin Ageing: Natural Weapons and Strategies. BMC Complement Altern Med. 2013; 2013: 27248. [CrossRef] [PubMed].
  15. Rittie L, Fisher GJ. UV-light-induced signal cascades and skin aging. Ageing Res Rev. 2002; 1(4): 705-20. [CrossRef] [PubMed].
  16. Papakonstantinou E, Roth M, Karakiulakis G. Hyaluronic acid: a key molecule in skin aging. Dermatoendocrinol. 2012 Jul 1; 4(3): 253-8. [CrossRef] [PubMed].
  17. Tammi R, Pasonen-Seppanen S, Kolehmainen E, Tammi M. Hyaluronan synthase induction and hyaluronan accumulation in mouse epidermis following skin injury. J Invest Dermatol. 2005 May; 124(5): 898-905. [CrossRef] [PubMed].
  18. West MD, Pereira-Smith OM, Smith JR. Replicative senescence of human skin fibroblasts correlates with a loss of regulation and over expression of collagenase activity. Exp Cell Res. 1989; 184(1): 138-47. [CrossRef] [PubMed].
  19. Limtrakul P, Yodkeeree S, Thippraphan P. Anti-aging and tyrosinase inhibition effects of Cassia fistula flower butanolic extract. BMC Complement Altern Med. 2016; 16: 497. [CrossRef].
  20. Van Doren SR. Matrix metalloproteinase interactions with collagen and elastin. Matrix biology: J Inter Soc Matrix Biol. 2015; 44-46: 224-31. [CrossRef].
  21. Antonicelli F, Bellon G, Debelle L, Hornebeck W. Elastin-elastases and inflamm-aging. Curr Top Dev Biol. 2007; 79: 99-155. [CrossRef] [PubMed].
  22. Sundararaj KP, Samuvel DJ, Li Y, Sanders JJ, Lopes-Virella MF, Huang Y. Interleukin-6 released from fibroblasts is essential for up-regulation of matrix metalloproteinase-1 expression by U937 macrophages in coculture: cross-talking between fibroblasts and U937 macrophages exposed to high glucose. J Biol Chem. 2009 May 15; 284(20): 13714-24. [CrossRef] [PubMed].
  23. Schönbeck U, Mach F, Libby P. Generation of Biologically Active IL-1β by Matrix Metalloproteinases: A Novel Caspase-1-Independent Pathway of IL-1β Processing. J Immunol. 1998 Oct 1; 161(7): 3340-46. [PubMed].
  24. Choi YA, Lee DJ, Lim HK, Jeong JH, Sonn JK, Kang SS et al. Interleukin-1β stimulates matrix metalloproteinase-2 expression via a prostaglandin E2-dependent mechanism in human chondrocytes. Exper Molec Med. 2004 Jun; 36(3): 226-32. [CrossRef] [PubMed].
  25. Cheng CY, Kuo CT, Lin CC, Hsieh HL, Yang CM. IL-1beta induces expression of matrix metalloproteinase-9 and cell migration via a c-Src-dependent, growth factor receptor transactivation in A549 cells. British J Pharmacol. 2010 Aug 7; 160(7): 1595-1610. [CrossRef] [PubMed].
  26. Suzuki M, Hashizume M, Yoshida H, Shiina M, Mihara M. IL-6 and IL-1 synergistically enhanced the production of MMPs from synovial cells by up-regulating IL-6 production and IL-1 receptor I expression. Cytokine. 2010 Aug; 51(2): 178-83. [CrossRef] [PubMed].
  27. Robinson S, Scott K, Balkwill F. Chemokine stimulation of monocyte matrix metalloproteinaseâ€9 requires endogenous TNFâ€Î±. Eur J Immunol. 2002 Feb; 32(2): 404-12. [CrossRef] [PubMed].
  28. Knott A, Reuschlein K, Mielke HV. Natural Arctium lappa fruit extract improves the clinical signs of aging skin. J Cosm Dermatol. 2008 Dec; 7(4): 281-89. [CrossRef] [PubMed].
  29. Bhattamisra SK, Hooi LP, Shyan LP, Chieh LB, Candasamy M, Sahu PS. Effect of geraniol and clarithromycin combination against gastric ulcers induced by acetic acid and Helicobacter pylori in rats. Phcog Res. 2019; 11(4): 356-62. [CrossRef] [Link].
  30. Wang J, Su B, Zhu H, Chen C, Zhao G. Protective effect of geraniol inhibits inflammatory response, oxidative stress and apoptosis in traumatic injury of the spinal cord through modulation of NF-κB and p38 MAPK. Exper Therap Med. 2016 Dec; 12(6): 3607-13. [CrossRef] [PubMed].
  31. Lei Y, Fu P, Jun X, Cheng P. Pharmacological Properties of Geraniol - A Review. Pl Med. 2019; 85(1): 48-55. [CrossRef] [PubMed]. Epub 2018 Oct 11.
  32. Huang Y, Yang XL, Ni YH, Xu ZM. Geraniol suppresses proinflammatory mediators in phorbol 12-myristate 13-acetate with A23187-induced HMC-1 cells. Drug Des Devel Ther. 2018 Sep 11; 12: 2897-2903. [CrossRef] [PubMed].
  33. Chen W, Viljoen AM. Geraniol - A review of a commercially important fragrance material. South Afr J Bot. 2010; 76(4): 643-51. [CrossRef].
  34. Sinha S, Jothiramajayam M, Ghosh M, Mukherjee A. Evaluation of toxicity of essential oils palmarosa, citronella, lemongrass and vetiver in human lymphocytes. Food Chem Toxicol. 2014 Jun; 68: 71-7. [CrossRef] [PubMed].
  35. Raina V, Srivastava SK, Aggarwal KK, Syamasundar KV, Khanuja SPS. Essential oil composition of Cymbopogon martini from different places in India. Flavour Fragr J. 2008; 18(4): 312-15. [CrossRef].

Most read articles by the same author(s)

PDF
HTML
XML (Portuguese)
Save bookmark

Author(s)

  • Carlos Rocha Oliveira
    Anhembi Morumbi University, Health Sciences. Rua Doutor Almeida Lima, s/n, Mooca, CEP 03164-000, São Paulo, SP, Brazil
  • Leonardo Mendes Bella
    Anhembi Morumbi University, Health Sciences. Rua Doutor Almeida Lima, s/n, Mooca, CEP 03164-000, São Paulo, SP, Brazil.
  • Marília Cristina Duarte
    University of Mogi das Cruzes, Center for Biomedical Sciences, Laboratory of Plant Systematics. Avenida Dr. Cândido Xavier de Almeida e Souza, 200, Centro Cívico, CEP 08780-911, Mogi das Cruzes, SP, Brazil.
  • Rodolfo de Paula Vieira
    University Brazil, Campus Itaquera. Rua Carolina Fonseca, 235, Vila Santana, CEP 08230-030, São Paulo, SP, Brazil.

Metrics

  • Article viewed 490 time(s)

How to Cite

1.
Brazilian essential oil of Cymbopogon martini (Poaceae): positive effects on inflammation-induced human fibroblasts and skin aging. Rev Fitos [Internet]. 2021 Dec. 17 [cited 2024 Oct. 30];15(4):444-55. Available from: https://teste.revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1127
Crossref
Scopus
Google Scholar
Europe PMC

1. DIREITOS CEDIDOS - A cessão total não exclusiva, permanente e irrevogável dos direitos autorais patrimoniais não comerciais de utilização de que trata este documento inclui, exemplificativamente, os direitos de disponibilização e comunicação pública da OBRA, em qualquer meio ou veículo, inclusive em Repositórios Digitais, bem como os direitos de reprodução, exibição, execução, declamação, recitação, exposição, arquivamento, inclusão em banco de dados, preservação, difusão, distribuição, divulgação, empréstimo, tradução, dublagem, legendagem, inclusão em novas obras ou coletâneas, reutilização, edição, produção de material didático e cursos ou qualquer forma de utilização não comercial.

2. AUTORIZAÇÃO A TERCEIROS - A cessão aqui especificada concede à FIOCRUZ - FUNDAÇÃO OSWALDO CRUZ o direito de autorizar qualquer pessoa – física ou jurídica, pública ou privada, nacional ou estrangeira – a acessar e utilizar amplamente a OBRA, sem exclusividade, para quaisquer finalidades não comerciais, nos termos deste instrumento.

3. USOS NÃO COMERCIAIS - Usos não comerciais são aqueles em que a OBRA é disponibilizada gratuitamente, sem cobrança ao usuário e sem intuito de lucro direto por parte daquele que a disponibiliza e utiliza.

4. NÃO EXCLUSIVIDADE - A não exclusividade dos direitos cedidos significa que tanto o AUTOR como a FIOCRUZ - FUNDAÇÃO OSWALDO CRUZ ou seus autorizados poderão exercê-los individualmente de forma independente de autorização ou comunicação, prévia ou futura.

5. DIREITOS RESERVADOS - São reservados exclusivamente ao(s) AUTOR(es) os direitos morais sobre as obras de sua autoria e/ou titularidade, sendo os terceiros usuários responsáveis pela atribuição de autoria e manutenção da integridade da OBRA em qualquer utilização. Ficam reservados exclusivamente ao(s) AUTOR(es) e/ou TITULAR(es) os usos comerciais da OBRA incluída no âmbito deste instrumento.

6. AUTORIA E TITULARIDADE - O AUTOR declara ainda que a obra é criação original própria e inédita, responsabilizando-se integralmente pelo conteúdo e outros elementos que fazem parte da OBRA, inclusive os direitos de voz e imagem vinculados à OBRA, obrigando-se a indenizar terceiros por danos, bem como indenizar e ressarcir a FIOCRUZ - FUNDAÇÃO OSWALDO CRUZ de eventuais despesas que vierem a suportar, em razão de qualquer ofensa a direitos autorais ou direitos de voz ou imagem, principalmente no que diz respeito a plágio e violações de direitos.

7. GRATUIDADE - A cessão e autorização dos direitos indicados e estabelecidos neste Instrumento será gratuita, não sendo devida qualquer remuneração, a qualquer título, ao autor e/ou titular, a qualquer tempo.

Report an error