Brazilian essential oil of Cymbopogon martinii: positive effects on inflammation-induced human fibroblasts and skin aging

Dr. Carlos Rocha Oliveira
OrcID
Dr. Leonardo Mendes Bella
OrcID
Dr. Rodolfo de Paula Vieira
OrcID
Drª Marília Cristina Duarte
OrcID

    Dr. Carlos Rocha Oliveira

    Anhembi Morumbi University

    OrcID https://orcid.org/0000-0001-8634-2850

    Farmacêutico-Bioquímico, Mestre em Farmacologia e Doutor em Biotecnologia. Pós-doutorado pelo Instituto de Farmacologia e Biologia Molecular da UNIFESP. Professor do curso de Medicina da Universidade Anhembi Morumbi em São José dos Campos-SP e Professor orientador no Programa de Pós-Graduação em Engenharia Biomédica (UNIFESP) em São José dos Campos-SP. Atualmente lidera o Grupo de Pesquisa e Fitocomplexos e Sinalização celular (DGP/CNPq) e é coordenador do Comitê de Ética em Pesquisa da Universidade Anhembi Morumbi (CEP-UAM). Desenvolve projetos na área de Farmacologia, com ênfase em Farmacologia de Produtos Naturais, atuando principalmente nos seguintes temas: sinalização celular e modo de ação de drogas, atividade antibacteriana e antioxidante de produtos naturais e fitoterápicos. Tem experiência no desenvolvimento de projetos em associação com a indústria farmacêutica, alimentícia e de cosméticos. 

    Dr. Leonardo Mendes Bella

    Anhembi Morumbi University

    OrcID https://orcid.org/0000-0002-4099-5597

    Possui graduação em Farmácia e Bioquímica pela Universidade Federal de Juiz de Fora (2012), mestrado em Farmácia (Fisiopatologia e Toxicologia) pela Universidade de São Paulo (2014) e doutorado em Farmácia (Fisiopatologia e Toxicologia) pela Universidade de São Paulo (2018) e pós-graduado em microbiologia (2020) na Faculdade Oswaldo Cruz (FOC). Atualmente é docente da Escola da Saúde da Universidade Anhembi Morumbi.

    Dr. Rodolfo de Paula Vieira

    Evangelical University of Goiás

    OrcID https://orcid.org/0000-0001-6379-1143

    Graduated in Physical Education (Univap 1998-2001), Master's degree in Biological Sciences (Univap 2001-2003), Doctorate in Pathology (CAPES Scholarship, Faculty of Medicine USP 2004-2007) and Post-doctorate in Physical Activity and Pulmonary Immunopathology (FAPESP Scholarship , Faculty of Medicine USP 2008-2009), Post-doctorate in Asthma Immunology (Deutsche Forchungsgemeinschaft Contract 2009-2010) from the Albert-Ludwigs University of Freiburg - Germany, Post-doctorate in Physical Activity and Pulmonary Immunopathology (European Respiratory Award Scholarship Society/Marie Curie Foundation/European Union 2010-2011). He was a guest researcher at the Department of Pulmonology at the Albert-Ludwigs University of Freiburg in Germany (June 2010 to May 2011) and an associate researcher in the same department (June 2011 to December 2011). He is the researcher responsible for the project "Effects of aerobic physical training on lung changes induced by different products of atmospheric pollution" financed by MCT / CNPq, which is part of the (INCTs-National Institutes of Science and Technology). Since April/2014 he has been the author and responsible for the Young Researcher Project FAPESP 2012/15165-2 entitled "Role of purinergic signaling and SOCS-JAK-STAT signaling in the anti-inflammatory effects of aerobic training in experimental models of asthma and in asthmatic individuals" , from where he obtained resources to set up the Pulmonary and Exercise Immunology Laboratory (LABPEI) in the city of São José dos Campos. He is currently director of the Brazilian Institute of Teaching and Research in Pulmonary and Exercise Immunology (IBEPIPE). He is coordinator of LABPEI (www.labpei.com.br), which is the functional arm of IBEPIPE, developing clinical and experimental research work, focusing on cellular and molecular immunological aspects involved in both the pathophysiology of lung diseases and the effects of physical exercise in lung diseases. Furthermore, the laboratory's research focus includes research in the area of exercise immunology, which is carried out with professional and amateur athletes and different sports teams. Advisor of the master's and doctorate programs in Human Movement Sciences and Rehabilitation at UNIFESP (and collaborating professor) and the master's and doctorate programs in Human Movement and Rehabilitation at Unievangélica and also the professional master's degree in Pharmaceutical Sciences at Unievangélica. He has been a CNPq level 2 research productivity fellow since 2016. Web of Science ResearcherID: E-9315-2011.

    Drª Marília Cristina Duarte

    University of Mogi das Cruzes

    OrcID https://orcid.org/0000-0002-1257-3389

    Doutora e mestre em Biodiversidade Vegetal e Meio Ambiente, na área de sistemática vegetal, pelo Instituto de Botânica de São Paulo. Atualmente, é pesquisadora e professora em nível de graduação e pós graduação na Universidade de Mogi das Cruzes (UMC), bem como curadora do Herbarium Mogiense (HUMC). Tem experiência na área de Botânica, com ênfase em Sistemática de Espermatófitas,em especial da familia Malvaceae, atuando principalmente em inventários florísticos e projetos institucionais. tendo publicado artigos científicos, capítulos de livros e participado de Congressos Nacionais e Internacionais de Botânica.

Palavras-chave

Cymbopogon martini
Anti-inflammatory
Anti-aging
Hyaluronic acid
Collagen

Resumo

This study evaluated the effects of essential oil from Cymbopogon martinii (CMEO) on lipopolysaccharide (LPS)-stimulated human fibroblast cells. Samples of CMEO were collected in Monte Verde, Minas Gerais, Brazil. The fibroblasts were cultured and stimulated by LPS (1 μg/mL), and incubated for 24 h at 37°C. The cytotoxicity of CMEO was evaluated by MTT assay and collagen concentration by Sirius red. Collagenase activity, hyaluronic acid, and the concentrations of IL-1β; IL-6; MCP-1 (CCL2), and MIP-1-α (CCL3) were evaluated by ELISA assay. The effect of CMEO on the expression of mRNA and secretion of MMP-1, MMP-2, and MMP-9 enzymes were evaluated by RT-qPCR and ELISA, respectively. CMEO was cytotoxic against fibroblasts, in which 10 μg/mL inhibited 50% of cell viability. When treated with CMEO, the fibroblasts produced more collagen and hyaluronic acid compared to control cells. When stimulated by LPS, fibroblasts exhibited higher production of IL-6, IL-1β, MCP-1, and MIP-1α compared to control cells. However, the treatment of fibroblasts with CMEO reduces cytokines secretion and enzyme expression. The study showed that CMEO modulates inflammation mediators and reduces metalloproteinase mRNA and secretion levels, making it a promising candidate for anti-aging and wound healing treatments.

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Brazilian essential oil of Cymbopogon martinii: positive effects on inflammation-induced human fibroblasts and skin aging. Rev Fitos [Internet]. 6º de junho de 2024 [citado 30º de outubro de 2024];18:e1130. Disponível em: https://teste.revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1130
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