In vitro antimicrobial activity of Alpinia zerumbet and A. purpurata nonpolar fraction of leaf extract

Cristiane Pimentel Victorio
Davi Oliveira e Silva
Daniela Alviano
Celuta Alviano
Ricardo Machado Kuster
Celso Luiz Salgueiro Lage

    Cristiane Pimentel Victorio

    Centro Universitário Estadual da Zona Oeste (UEZO), Colegiado de Ciências Biológicas e da Saúde - CCBS. Avenida Manuel Caldeira de Alvarenga, 1203 Campo Grande, CEP 23070-200, Rio de Janeiro, RJ, Brasil.

    holds a degree in Biological Sciences from the State University of Rio de Janeiro (2000) and a master's and doctorate in Biological Sciences from the Biophysics Institute of the Federal University of Rio de Janeiro (2004/2008). In the area of ​​Education, she completed a post-graduate course (latu-sensu) talking about the use of trails in Botany Education, by UERJ (2002) and has projects for popularization and environmental education in schools in the city of Rio de Janeiro. She has experience in the field of Botany, with an emphasis on Plant and Environmental Biotechnology, working on the following topics: plant tissue culture, plant anatomy and physiology, phytochemistry and phytoremediation. During the master's degree, she did studies relating biophysical aspects, such as light, UV radiation and hormones and plant development, anatomy and phytochemistry. In the doctorate, he evaluated the production of special metabolites in vitro, using tissue culture and phytochemical techniques: flavonoids and essential oils, in addition to biological activities of plant extracts. She is currently an adjunct professor of Plant Biology and Biotechnology at UEZO-Rio de Janeiro and is a member of the teaching group of the Professional Master in Environmental Science and Technology.

    Davi Oliveira e Silva

    Universidade Federal de Ouro Preto (UFOP), Department of Biodiversity, Evolution and Environment-DEBIO, Institute of Exact and Biological Sciences (ICEB), Morro do Cruzeiro campus, CEP 35400-000, Ouro Preto, MG, Brazil.

    Graduated in Biological Sciences: Microbiology and Immunology from the Federal University of Rio de Janeiro-UFRJ (2006), MASTER in Plant Biotechnology - Decan of CCS - UFRJ (2009) and PhD in Plant Biotechnology - Decan of CCS - UFRJ (2013). He is currently a Biologist at the Federal University of Ouro Preto-UFOP where he is responsible for analysis in High Performance Liquid Chromatography (HPLC) of environmental samples from DEBIO-ICEB III, as well as monitoring maintenance of analytical equipment. Has experience in the area of Applied Microbiology, with emphasis on Research of new antimicrobial agents, acting mainly on the following themes: Cocos nucifera L., Aristolochia cymbifera, Plant Extracts, Essential Oil, Biological Activity, Phytochemical Analysis and Analysis of Environmental Samples.

    Daniela Alviano

    Paulo de Góes Institute of Microbiology, Federal University of Rio de Janeiro, Av. Carlos Chagas Filho, s / n, 21941-902 Rio de Janeiro-RJ, Brazil

    Celuta Alviano

    Paulo de Góes Institute of Microbiology, Federal University of Rio de Janeiro, Av. Carlos Chagas Filho, s / n, 21941-902 Rio de Janeiro-RJ, Brazil

    Ricardo Machado Kuster

    Federal University of Espírito Santo (UFES), Department of Chemistry, Av. Fernando Ferrari, 514, Goiabeiras, CEP 29075-910, Vitória, ES, Brazil

    Graduated from the Faculty of Pharmacy and Biochemistry of Espirito Santo (1987), master's degree in Chemistry of Natural Products from the Federal University of Rio de Janeiro (1991) and doctorate in Chemistry of Natural Products from the Federal University of Rio de Janeiro (1994). He is currently a professor and researcher in the Department of Chemistry at the Federal University of Espírito Santo, working in the research of natural products for application in the petroleum industry and chromatography of petroleum and derivatives.

    Celso Luiz Salgueiro Lage

    National Institute of Industrial Property (INPI), DICOD - Academia. Praça Mauá 7, Sala 1012, Centro, CEP 20081-240, Rio de Janeiro, RJ, Brazil

    Graduated in Physics from the Federal University of Rio de Janeiro (1984), Master's in Biological Sciences (Biophysics) from the Federal University of Rio de Janeiro (1989) and PhD in Biological Sciences (Biophysics) from the Federal University of Rio de Janeiro (1995) . Post-doctorate in the Department of Scientific and Technological Policy of the IG of UNICAMP. He is currently Senior Specialist in Industrial Property in Biotechnology at INPI. He has experience in Plant Biotechnology, with an emphasis on Tissue Culture, and Intellectual Property in Biotechnology.

Keywords

Antifungal activity
Zingiberaceae
Agar drop diffusion
Filamentous fungi
Yeast

Abstract

This study aimed to evaluate the in vitro antimicrobial activity of fractions of leaf hydroalcoholic crude extracts: hexane, dichloromethane, ethyl acetate and butanolic of Alpinia zerumbet and A. purpurata by the agar drop diffusion method in order to screen the main compounds involved in antimicrobial activity. Leaves of A. zerumbet (Pers.) B.L. Burtt et R.M. Sm. and A. purpurata (Vieill) K. Schum adult plants were collected and then dried and macerated in 70% ethanol. Leaf extracts were further partitioned using solvents in increasing polarity. Dichloromethane fractions were analyzed by gas chromatography/mass spectrometry (GC/MS). The major compound in the dichloromethane fraction of both species was the hexadecanoic acid (palmitic acid). By drop diffusion assay, the antimicrobial activity of crude extract, as well as fractions of hexane, dichloromethane, ethyl acetate and butanolic, was evaluated against pathogenic bacteria and fungi. No bacteria were inhibited. However, the dichloromethane fraction exhibited promising antifungal activity against the following fungi tested: Cryptococcus neoformans, Fonsecaea pedrosoi, Trichophytoon rubrum, Microsporium canis and M. gypseum.

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Author(s)

  • Cristiane Pimentel Victorio
    Centro Universitário Estadual da Zona Oeste (UEZO), Colegiado de Ciências Biológicas e da Saúde - CCBS. Avenida Manuel Caldeira de Alvarenga, 1203 Campo Grande, CEP 23070-200, Rio de Janeiro, RJ, Brasil.
  • Davi Oliveira e Silva
    Universidade Federal de Ouro Preto (UFOP), Department of Biodiversity, Evolution and Environment-DEBIO, Institute of Exact and Biological Sciences (ICEB), Morro do Cruzeiro campus, CEP 35400-000, Ouro Preto, MG, Brazil.
  • Daniela Alviano
    Paulo de Góes Institute of Microbiology, Federal University of Rio de Janeiro, Av. Carlos Chagas Filho, s / n, 21941-902 Rio de Janeiro-RJ, Brazil
  • Celuta Alviano
    Paulo de Góes Institute of Microbiology, Federal University of Rio de Janeiro, Av. Carlos Chagas Filho, s / n, 21941-902 Rio de Janeiro-RJ, Brazil
  • Ricardo Machado Kuster
    Federal University of Espírito Santo (UFES), Department of Chemistry, Av. Fernando Ferrari, 514, Goiabeiras, CEP 29075-910, Vitória, ES, Brazil
  • Celso Luiz Salgueiro Lage
    National Institute of Industrial Property (INPI), DICOD - Academia. Praça Mauá 7, Sala 1012, Centro, CEP 20081-240, Rio de Janeiro, RJ, Brazil

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In vitro antimicrobial activity of Alpinia zerumbet and A. purpurata nonpolar fraction of leaf extract. Rev Fitos [Internet]. 2021 Jun. 30 [cited 2024 Oct. 30];15(2):136-43. Available from: https://teste.revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1037
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